Investigation of the Correlation between the Antioxidant, Antimicrobial Activity and the Content of Phenolic Compounds of St. John`s Wort (Hypericum perforatum L.) Liquid Extracts

Abstract

Infection diseases is a worldwide important problem for medicine and pharmacy. The purpose of work was study the total content of some biologically active substances (BAS), determine antimicrobial, anti-fungi and antioxidant activity of obtained St. John`s Wort extracts, and study a correlation analysis between the content of natural compounds and antimicrobial/antifungal and antioxidant activity. Results demonstrate the highest amount of polyphenols, flavonoids, anthraquinone derivatives and organic acids were 1.37±0.02, 0.66±0.01, 0.05±0.005 and 0.66±0.005% in 60% EtOH extract, respectively. The hydroxycinnamic acids was dominated in 40% extract (0.72±0.01). The most potent antioxidant property possessed 60% EtOH extract of John`s Wort herb. The high correlation was found between the total polyphenols, flavonoids and antioxidant/antimicrobial/antifungal effects against all Gram-positive, Gram-negative bacterial strains and the fungus C. albicans. These findings have showed the great potential of St. John’s wort in the development and creation of new medicines with antimicrobial, antioxidant and antifungal effects that are not inferior to, and even superior to, the effects of synthetic analogues.