Apoptosis-mediated antitumor activity of the ethanolic extract of Calotropis procera flowers: in vitro testing on the MG63 human osteosarcoma cell line
DOI:
https://doi.org/10.60988/p.v37i2S.158Keywords:
apoptosis; cancer; osteosarcoma; MG63; Calotropis proceraAbstract
Developing cancer therapeutics that selectively target malignant cells without harming healthy tissue or encountering resistance remains a central challenge in oncology. This study aimed at evaluating the effects of an ethanol (70%) extract of Calotropis procera L. flowers on the osteosarcoma MG63 cell line. Following serial dilution, the extract was assessed by using a multi-parameter cytotoxicity assay in order to measure cell viability (valid cell count), total nuclear intensity, mitochondrial membrane potential, cell membrane permeability, and cytochrome c release. These parameters were analysed via high-content screening (HCS) in order to determine the extract’s therapeutic potential. The HCS results demonstrated that treatment with 200 μg/mL of the Calotropis procera flower extract can produce a highly significant effect across all five of the aforementioned parameters in MG63 cells.
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