Chemometric Assisted Uv-Vis Spectroscopic Study of Photostability of Some Beta-Blockers With Multivariate Curve Resolution-alternating Least Square (Mcr-Als) Method Using Soft and Hard Modelling Approach.
DOI:
https://doi.org/10.60988/pj.v35i4.27Abstract
The present work focuses on the use of simple UV-Vis spectrophotometric methods along with chemometric tools to understand the photodegradation and degradation kinetics of five Beta blockers namely atenolol, bisoprolol, metoprolol, propranolol, and sotalol. All the molecules were exposed to sunlight for 24 hours and UV spectra were recorded between 200 -400 nm for a specific interval of time. Amber colour and transparent glass material were used to study the penetration effect of sunlight. The recorded UV absorbance spectra were subjected to Multivariate curve resolution alternating least square method using soft and hard modelling constraints to get an understanding of the number of pure components and their concentration in the reaction mixture. Atenolol, Bisoprolol, Metoprolol, and Sotalol were found to be stable even after exposure to direct sunlight, but Propranolol shows photodegradation through a two-step mechanism Photodegradation kinetics was studied for Propranolol using kinetic constrain under study conditions. ALS optimization shows percentage lack of fit value was found to be 0.0073 with 99.98% variance explained. The standard deviation of residual against experimental data was found to be 0.0129 which suggests the statistical significance of the developed model. The rate constants of the first (k1) and second (k2) steps were calculated as 0.00315 h−1 and 0.0149 h−1 respectively.
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