Development of the composition and technology for producing green tea leaf extract with antioxidant effects based on theoretical and practical research

Abstract

The aim of the work was to develop the composition and technology for obtaining green tea leaf extract with antioxidant effects based on theoretical and practical research. Theoretical studies of the antioxidant effect were carried out by molecular docking with the AutoDockTools 1.4.6 program, the quantitative content of biologically active substances (BAS) was determined by HPLC-UV, and the level of antioxidant action was studied by the potentiometric method. In in sillico studies, epicatechin, (+)-catechin and epigallocatechin inhibit the active sites of pro-oxidant enzymes more effectively than epigallocatechin-3-O-gallate, epicatechin-3-O-gallate and gallic acid. The level of antioxidant activity of epigallocatechin-3-O-gallate was the highest (), and gallic acid was the lowest (). After hydrolysis of green tea leaf extract, the content of epigallocatechin increased by 46%, epicatechin by 64%, and gallic acid by 91%, while gallic acid esters were not identified. The antioxidant interaction of combinations of gallic acid with epicatechin and with epigallocatechin is antagonistic. During the study, a composition and technology for obtaining green tea leaf extract was developed, including the destruction of esters and the removal of gallic acid.