Method development and validation for the spectrophotometric determination of dihydroquercetin in capsule formulation
DOI:
https://doi.org/10.60988/p.v37i4.123Keywords:
Dihydroquercetin, Taxifolin, antioxidant, analytical method, UV spectroscopy, Visible spectroscopy, colorimetry, quantitationAbstract
Dihydroquercetin is a naturally occurring dihydroflavone found to exist abundantly in woods of trees like larch. The other name for dihydroquercetin, which is commonly used, is Taxifolin. Dihydroflavone is a subclass of flavanols, which are a type of polyphenol. It is a bioactive component found in various food items, including herbal tea, fruits and milk thistle. Dihydroquercetin is reported to have antioxidant properties and so used in the form of health supplement. Numerous studies have reported the anti-inflammatory properties of taxifolin, its potential use in cancer treatment, and its application in the treatment of liver diseases, cardiovascular diseases, and neurodegenerative disorders. Additionally, it has been claimed to have antimicrobial actions. With the increase in potential activities for taxifolin, the possibilities of formulation development for taxifolin can be significantly enhanced. For the quantitative estimation of dihydroquercetin, the present study focused on the development of a simple, cost cost-effective method using UV and visible spectroscopy, which was validated according to the ICH guideline. The solvent used for the analysis is methanol 99% for both the UV and Visible (colorimetric) methods. Using the method, linearity for dihydroquercetin was found to exist in the concentration range of 1 to 5 µg/mL for the UV method and in the range of 80 to 120 µg/mL for the colorimetric methods. The parameters are also statistically validated to prove their significance. Various validation parameters including accuracy, precision, linearity, range, and limits of measurement, such as limit of detection and limit of quantitation, were studied. The accuracy and precision of the developed methods are tested in terms of percentage relative standard deviation for the observations, which were found to be less than 2%. The assay values for the finished formulations in capsules form were found to lie between the range of 99.7 and 99.8%w/w for UV and colorimetric methods. The correlation coefficient is calculated for the observations from the linearity plot and was found to be near to 1 for both methods. Thus, the developed methods can be employed as analytical methods for ensuring the quality of the marketed formulations.
References
Abhijit Das, Ratna Baidya, Tania Chakraborty, Akash Kumar Samanta, Souvik Roy, Pharmacological basis and new insights of taxifolin: A comprehensive review, Biomedicine & Pharmacotherapy, Volume 142, 2021, 112004, ISSN 0753-3322, https://doi.org/10.1016/j.biopha.2021.112004.
National Center for Biotechnology Information. PubChem Compound Summary for CID 439533, Taxifolin. https://pubchem.ncbi.nlm.nih.gov/compound/dihydroquercetin. Accessed Sept. 10, 2024.
Shafeeq Ur Rehman, Furqan Shafqat, Bisal Fatima, Muhammad Naveed Nawaz, Kamal Niaz, Chapter 4 - Flavonoids and other polyphenols against SARS-CoV-2, Editor(s): Kamal Niaz, Application of Natural Products in SARS-CoV-2, Academic Press, 2023, Pages 83-123, ISBN 9780323950473
Micek I, Nawrot J, Seraszek-Jaros A, Jenerowicz D, Schroeder G, Spiżewski T, Suchan A, Pawlaczyk M, Gornowicz-Porowska J. Taxifolin as a Promising Ingredient of Cosmetics for Adult Skin. Antioxidants (Basel). 2021 Oct 15;10(10):1625. doi: 10.3390/antiox10101625. PMID: 34679758; PMCID: PMC8533573.
Topal F, Nar M, Gocer H, Kalin P, Kocyigit UM, Gülçin İ, Alwasel SH. Antioxidant activity of taxifolin: an activity-structure relationship. J Enzyme Inhib Med Chem. 2016 Aug;31(4):674-83. doi: 10.3109/14756366.2015.1057723. Epub 2015 Jul 6. PMID: 26147349.
Liu, Z.; Qiu, D.; Yang, T.; Su, J.; Liu, C.; Su, X.; Li, A.; Sun, P.; Li, J.; Yan, L.; et al. Research Progress of Dihydroquercetin in the Treatment of Skin Diseases. Molecules 2023, 28, 6989. https://doi.org/10.3390/molecules28196989
Kundrapu DB, Chaitanya AK, Manaswi K, Kumari S, Malla R. Quercetin and taxifolin inhibits TMPRSS2 activity and its interaction with EGFR in paclitaxel-resistant breast cancer cells: An in silico and in vitro study. Chem Biol Drug Des. 2024 Aug;104(2):e14600. doi: 10.1111/cbdd.14600. PMID: 39075030.
Yorito Hattoria, Satoshi Saitoa, Yuriko Nakaokub, Soshiro Ogatab, Masashi Hattoric, Mio Nakatsujid, Kunihiro Nishimurab and Masafumi Iharaa, Taxifolin for Cognitive Preservation in Patients with Mild Cognitive Impairment or Mild Dementia, Journal of Alzheimer’s Disease 93 (2023) 743–754, DOI 10.3233/JAD-221293.
Inoue T, Fu B, Nishio M, Tanaka M, Kato H, Tanaka M, Itoh M, Yamakage H, Ochi K, Ito A, Shiraki Y, Saito S, Ihara M, Nishimura H, Kawamoto A, Inoue S, Saeki K, Enomoto A, Suganami T, Satoh-Asahara N. Novel Therapeutic Potentials of Taxifolin for Obesity-Induced Hepatic Steatosis, Fibrogenesis, and Tumorigenesis. Nutrients. 2023 Jan 10;15(2):350. doi: 10.3390/nu15020350. PMID: 36678220; PMCID: PMC9865844.
Kim, YJ., Nam, T.G., Lee, I. et al. Development and validation of analytical HPLC for phenolics in Pinus densiflora bark extract. Food Sci Biotechnol (2024). https://doi.org/10.1007/s10068-024-01616-x
Khalisanni Khalid, Ruzaina Ishak, Zaira Zaman Chowdhury, Chapter 15 - UV–Vis spectroscopy in non-destructive testing, Editor(s): Akira Otsuki, Seiko Jose, Manasa Mohan, Sabu Thomas, Non-Destructive Material Characterization Methods, Elsevier, 2024, Pages 391-416, ISBN 9780323911504, https://doi.org/10.1016/B978-0-323-91150-4.00021-5. (https://www.sciencedirect.com/science/article/pii/B9780323911504000215).
Gurdeep Chatwal, Sham K. Anand, Instrumental methods of Chemicals Analysis, 5th edition, Himalaya publishing house, New Delhi, 2002, 1.1-1.8.
https://database.ich.org/sites/default/files/Q2%28R1%29%20Guideline.pdf
