Use of Mass Spectrometry for the Determination of Prohibited Substances in Athletes (Anti-Doping)
DOI:
https://doi.org/10.60988/p.v36i2.57Abstract
The determination of prohibited substances and their metabolites as defined by the World Anti-Doping Agency (WADA) in athletes is carried out by analytical methods based on Mass Spectrometry (MS). This technique is suitable for determining changes in metabolism during the initial control of anti-doping tests (‘’screening’’), while at the same time it is also useful in the field of metabolomics. The mass spectrometer is often combined by some chromatographic technique for the separation of substances, usually gas or liquid chromatography (GC-MS, HPLC-MS, LC-HRMS). Apart from them, a very promising technique is supercritical fluid chromatography (SFC-MS/MS), which is used for the separation of steroids’ metabolites in their glucuronide form.
Finally, the LC-MS/MS technique enables the discovery of new metabolites of diagnostic importance, helps to detect chronic drug and anabolic substance use, while also distinguish between doping and other uses/abuses of a drug. The GC-MS technique can help to prevent the potential finding of athletes who use nutritional supplements as positive for steroids and in eliminating risks from the purchase of health care products by the general public.
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