Latin Square Design For Selection of Excipients in the Development of the Composition of Medical Sponges Based on the Cryoliophylized Xenoderm
Keywords:
medical sponges, xenoderm powder, Latin squares design, drug technology, excipients.Abstract
Medical sponges are popular medical tools, which widely used in various fields of medicine to stop bleeding and for close wound surfaces. Therefore, the aim of our work was to study the selection of excipients in order to develop the composition and technology of medical sponges based on a material of biological origin - cryolyophilized xenoderm, which has wide practical application in combustiology.
The influence of three groups of factors on the technological parameters of 16 experimental series of the sponges was studied. The indicators: pH, appearance, water absorption, time of complete dissolution, In vitro degradation, thickness of sponges, and weight loss during drying.
The influence of factors and their levels on the quality indicators of the developed medical sponges is investigated. It is established that the influence of the type of polymer is decisive for all characteristics except for weight loss during drying, which is more influenced by the type of plasticizer. Using the generalized indicator - the desirability function, the optimal levels of factors within each factor are selected.
According to the results of the tests, it was found that the best values of technological indicators were medical sponges based on water extract obtained at 40° C, which included medical gelatin and propylene glycol.
References
Chen Z., Han L., Liu C., Du Y., Hu X., Du G., Shan C., Yang K., Wang C., Li M., Li F., Tian F. A rapid hemostatic sponge based on large, mesoporous silica nanoparticles and N-alkylated chitosan. Nanoscale, 10(43), 20234-45, 2018. doi: https://doi.org/10.1039/C8NR07865C
Rodrigues S.C., Salgado C.L., Sahu A., Garcia M.P., Fernandes M.H., Monteiro F.J. Preparation and characterization of collagen-nanohydroxyapatite biocomposite scaffolds by cryogelation method for bone tissue engineering applications. J. Biomed. Mater. Res. A, 101(4), 1080-94, 2013. doi: https://doi.org/10.1002/jbm.a.34394
Liu J.Y., Li Y., Hu Y., Cheng G., Ye E., Shen C., Xu F.J. Hemostatic porous sponges of cross-linked hyaluronic acid/cationized dextran by one self-foaming process. Mater. Sci. Eng. C. Mater. Biol. Appl, 83, 160-8, 2018. doi: https://doi.org/10.1016/j.msec.2017.10.007
Lytvynyuk S.O., Volkov K.S., Nebesna Z.M. Morphofunctional Modifications of Neurocytes of the Rat Hippocampal CA3 Area after Experimental Thermal Trauma. Neurophysiology, 48, 399-406, 2016. doi: https://doi.org/10.1007/s11062-017-9616-z
Bennett B.L., Littlejohn L. Review of new topical hemostatic dressings for combat casualty care. Military Medicine, 179(5), 497-514, 2014. doi: https://doi.org/10.7205/MILMED-D-13-00199
Marushchak M., Krynytska I., Milevska L., Miz A., Mialiuk O. The changes of activity of effector caspase cascade components in case of alimentary obesity in rats. BJMS, 16(2), 252-8, 2017. doi: https://doi.org/10.3329/bjms.v16i2.31280
Jeschke M.G., Van Baar M.E., Choudhry M.A., Chung K.K., Gibran N.S., Logsetty S. Burn injury. Nat. Rev. Dis. Primers, 6(1), 11, 2020. https://doi.org/10.1038/s41572-020-0145-5
Skakun L.N., Cimbaluk A.V. Experience in the use of lyophilized xenodermotransplant for the treatment of patients with frequently recurrent inflammation of the maxillary sinus in combination with alveolar fistula. Vestn. Otorinolaringol., 80(3). 45-6, 2015. (in Ukrainian)
Pavliuk, B., Chubka, M., Hroshovyi, T., Stechyshyn, I. Characteristics of structured medical hemostatic sponges as a medical devices for stop bleeding and for close the wound. Pol. Merkur. Lekarski, 48(288), 422-6, 2020.
Dean A.M. (2015). Experimental Design: Overview International Encyclopedia of the Social & Behavioral Sciences. Editor(s): James D. Wright, International Encyclopedia of the Social & Behavioral Sciences (Second Edition), Elsevier, pp 535-540.
Tempelman R.J. Experimental design and statistical methods for classical
and bioequivalence hypothesis testing with an application to dairy
nutrition studies. J. Anim. Sci.. 82 E-Suppl: E162-172, 2004.
Hroshovyi T.A., Martseniuk V.P., Kucherenko L.I., Vronska L.V., Hurieieva C.M. (2008). Mathematical planning of ex-periment in pharmacy/ Ternopil: Ternopil State Medical University. (in Ukrainian).
Darzuli N., Budniak L., Hroshovyi T. Selected excipients in oral solid dosage form with dry extract of pyrola Rotundifolia L. Int. J. App. Pharm. 11(6), 210-6, 2019. doi: https://doi.org/10.22159/ijap.2019v11i6.35282
Dalwadi C., Patel G. Implementation of “Quality by Design (QbD)” Approach for the Development of 5-Fluorouracil Loaded Thermosensitive Hydrogel. Curr. Drug Deliv., 13(4), 512-27, 2016. doi: https://doi.org/10.2174/1567201812666150817122506
Hu-Fan Song, Ai-Zheng Chen, Shi-Bin Wang, Yong-Qiang Kang, Shi-Fu Ye, Yuan-Gang Liu, Wen-Guo Wu. Preparation of Chitosan-based hemostatic sponges by supercritical fluid technology. Materials (7), 2459-73, 2014.
Imani R., Rafienia M., Emami S.H. Synthesis and characterization of glutaraldehyde-based crosslinked gelatin as a local hemostat sponge in surgery: an in vitro study. Biomed. Mater. Eng. 23(3), 211-24, 2013.
Pavliuk B.V., Melnyk Y.Y., Hroshovyi Т.А., Chubka М.B., Skorokhoda V.Y. Research of water extraction from xenoderm as an active pharmaceutical ingredient in drugs. Farm. Zh. (5), 42-50, 2020. (in Ukrainian) https://doi.org/10.32352/0367-3057.5.20.05
Vons B.V., Hroshovyi T.А. Pharmacological justification for the choice of active pharmaceutical ingredients for the local treatment of burns in a model of aseptic burns in rats. Med. Сlin. Сhem. (1), 55-62, 2019. (in Ukrainian).
